Insecticidal,antimicrobial and antioxidant activities of bulb extracts of Allium sativum

来源 :Asian Pacific Journal of Tropical Medicine | 被引量 : 0次 | 上传用户:chu573346412
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Objective:To evaluate the insecticidal,antimicrobial and antioxidant activities of bulb extracts of Allium sativum(A.sativum).Methods:Dried bulbs of A.sativum were extracted with different solvents and evaluated for insecticidal,antimicrobial and antioxidant activities.Results: Aqueous and methanol extracts showed highest insecticidal activity(mortality rate of 81%and 64%respectively) against the larvae of Spodoptera litura(S.litura) at a concentration of 1000 ppm.With regard to antimicrobial activity,aqueous extract exhibited antibacterial activity against gram positive(Bacillus subtilis,Staphylococcus aureu,) and gram negative(Escherichia coli and Klebsiella pneumonia) strains and antifungal activity against Candida albicans.While methanol extract showed antimicrobial activity against all the tested micro organisms except two(Staphylococcus aureus and Candida albicans),the extracts of hexane,chloroform and ethyl acetate did not show any anti microbial activity.Minimum inhibitory concentration of aqueous and methanol extracts against tested bacterial and fungal strains was 100-150μg/mL. Antioxidant activity of the bulb extracts was evaluated in terms of inhibition of free radicals by 2.2’-diphenly-l-picrylhydrazyl.Aqueous and methanol extracts exhibited strong antioxidant activity(80%-90%of the standard).Conclusions:Antioxidant and antimicrobial activity of A.sativum against the tested organisms therefore,provides scientific basis for its utilization in traditional and folk medicine.Also,our results demonstrated the insecticidal efficacy of A. sativum against S.litura,a polyphagous insect. Objective: To evaluate the insecticidal, antimicrobial and antioxidant activities of bulb extracts of Allium sativum (A. sativum). Methods: Dried bulbs of A. sativum were extracted with different solders and evaluated for insecticidal, antimicrobial and antioxidant activities. Results: Aqueous and methanol extract showed highest insecticidal activity (mortality rate of 81% and 64% respectively) against the larvae of Spodoptera litura (S. litura) at a concentration of 1000 ppm. Due regard to antimicrobial activity, aqueous extract exhibited antibacterial activity against gram positive Bacillus subtilis, Staphylococcus aureu,) and gram negative (Escherichia coli and Klebsiella pneumonia) strains and antifungal activity against Candida albicans. Whilst methanol extract showed antimicrobial activity against all the tested micro organisms except two (Staphylococcus aureus and Candida albicans), the extracts of hexane, chloroform and ethyl acetate did not show any anti microbial activity. Minimal inhibitory conc entration of aqueous and methanol extracts against tested bacterial and fungal strains was 100-150 μg / mL. Antioxidant activity of the bulb extracts was evaluated in terms of inhibition of free radicals by 2.2’-diphenly-l-picrylhydrazyl. antioxidant activity (80% -90% of the standard) .Conclusions: Antioxidant and antimicrobial activity of A. sativum against the tested organisms therefore, scientific basis for its utilization in traditional and folk medicine. Also, our results demonstrated the insecticidal efficacy of A. sativum against S.litura, a polyphagous insect.
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