目的　探讨中国人非综合征性语前聋患者α -盖膜蛋白基因突变频率和特性。方法　收集非综合征性语前聋家系 34个 ,大部分来自湖南地区 ,共计 6 5例 ,散发患者 31例 ,健康对照组 10 0例。聚合酶链反应扩增α -盖膜蛋白基因的部分外显子 ,单链构象多态分析初筛可疑突变者 ,发现异常构象带后再行DNA测序。结果　在α-盖膜蛋白基因 9号和 18号外显子的SSCP检测中发现多个患者有异常构象带 ,测序证实为多态性改变。未发现导致非综合征性语前聋的突变。结论　目前在国人非综合征性语前聋患者中尚未检测到α -盖膜蛋白基因的致聋突变 ,该基因的其它外显子有待进一步的研究 Objective To investigate the frequency and characteristics of α - cambium gene mutation in Chinese nonsyndromic prelingual deafness patients. Methods 34 non-syndromic prelingual deaf families were collected, most of them from Hunan Province, with a total of 65 cases, 31 cases of sporadic cases and 100 cases of healthy controls. Polymerase chain reaction amplified part of exon of α - capped membrane protein gene, single - strand conformation polymorphism analysis, screening of suspicious mutation, discovery of abnormal conformation band and then DNA sequencing. Results In SSCP detection of exon 9 and exon 18 of the alpha-coat protein gene, many patients were found to have abnormal conformational bands, and the sequencing proved to be polymorphic. No mutations were found in non-syndromic prelingual deafness. Conclusion At present, no deafness mutation of α - CAP gene has been detected in Chinese non - syndromic prelingual deafness, and other exons of this gene need further study