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目的:建立超高效液相色谱(UPLC)法同时测定复方守宫散中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1含量的方法。方法:用Acquity BEH C18(2.1 mm×100 mm,1.7μm),乙腈-水为流动相,梯度洗脱,流速0.25 ml·min-1,柱温26℃,检测波长203 nm。结果:三七皂苷R1在0.053~0.53μg(r=0.999 8)、人参皂苷Rg1在0.067 5~0.675μg(r=0.999 8)、人参皂苷Re在0.061~0.61μg(r=0.999 6)、人参皂苷Rb1在0.066 3~0.662 5μg(r=0.999 8)范围内线性关系良好;平均回收率分别为97.96%,97.81%,97.18%,98.52%,RSD分别为1.69%,1.85%,2.73%,2.00%。结论:该方法快速、可靠、准确,可作为复方守宫散的质量控制方法。
Objective: To establish a method for simultaneous determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Fufang Shougong Powder by ultra performance liquid chromatography (UPLC). Methods: Acquity BEH C18 (2.1 mm × 100 mm, 1.7 μm) and acetonitrile-water were used as mobile phases. The flow rate was 0.25 ml · min-1 and the column temperature was 26 ℃. The detection wavelength was 203 nm. Results: The levels of notoginsenoside R1 in the range of 0.053-0.53μg (r = 0.999 8), ginsenoside Rg1 in 0.0675-0.675μg (r = 0.999 8), ginsenoside Re in 0.061-0.61μg (r = 0.999 6), ginseng The average recoveries were 97.96%, 97.81%, 97.18% and 98.52%, respectively, and the RSDs were 1.69%, 1.85%, 2.73% and 2.00% respectively for the range of 0.066 3 ~ 0.662 5μg (r = 0.999 8) %. Conclusion: The method is rapid, reliable and accurate and can be used as a quality control method for compound prescription.