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目的 研究血管内皮细胞生长因子（ ＶＥＧＦ） 与乙型肝炎（ 乙肝）分级（ Ｇ） 和分期（Ｓ） 的关系．方法 乙肝活检肝标本１５０ 例，用ＶＥＧＦ 单克隆抗体进行免疫组化（ＳＰ 法） 染色．结果 ＶＥＧＦ 在肝组织表达有胞浆型、膜窦型及窦内皮细胞型；ＶＥＧＦ 主要由肝细胞分泌，其次在肝窦内皮细胞、贮脂细胞、成肌纤维细胞，单形核细胞也示阳性． 无明显病变的血管内皮细胞及肝细胞ＶＥＧＦ 染色阴性． 提示随分级增高ＶＥＧＦ表达增强，其间有极显著性差异（ Ｐ＜ ０－０１） ；而Ｓ 低分值者ＶＥＧＦ 多示阳性表达，Ｓ 较高分值者其多为强阳性表达，两者有明显不同（ Ｐ＜ ０－０１） ．结论 ＶＥＧＦ 表达与乙肝Ｇ 和Ｓ 呈相关．
Objective To study the relationship between vascular endothelial growth factor (VEGF) and hepatitis B (G) and staging (S). Methods Hepatitis B biopsy liver specimens 150 cases, with VEGF monoclonal antibody immunohistochemistry (S P method) staining. Results The expression of VEGF in the liver tissue was cytoplasm, membrane sinus and sinus endothelial cell type. VEGF was mainly secreted by hepatocytes, followed by sinusoidal endothelial cells, fat storage cells, myofibroblasts and mononuclear cells. Vascular endothelial cells and hepatocytes without obvious pathological changes were negative for VEGF staining. These findings suggest that the expression of VEGF increased with the increase of grade, with a significant difference (P <0-01). However, the expression of VEGF in S low score group was mostly positive, while that in S higher score was mostly strongly positive Significantly different (P <0-01). Conclusion The expression of VEGF is correlated with G and S of hepatitis B virus.