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目的 明确肝脏微粒体中细胞色素P450酶活性与苦参中对抗CVB病毒的有效成分抗柯注射液药物剂量及用药时间的关系。方法 用定量RT-PCR法,在Begale犬长毒试验的不同时期(45、90、135 d),用不同剂量抗柯注射液(7.5、15、30 mg/kg)检测肝脏微粒体中细胞色素P450酶基因(β-actin/P450)的特异mRNA。结果 P450酶基因(β-actin/P450)特异信使核糖核酸mRNA随所用抗柯注射液剂量及用药时间而增加(P<0.01)。结论 P450的比值随药物剂量及时间延长而上升,可视为机体抵抗中毒而作出的一种适应性反应,可用于有关药物的毒理研究。
Objective To determine the relationship between cytochrome P450 enzyme activity in hepatic microsomes and the anti-CVB virus active ingredient, Kangke injection dose and duration of drug use, in Sophora flavescens. Methods Quantitative RT-PCR was used to detect cytochromes in liver microsomes at different time (45, 90, and 135 d) in the Begale canine poisoning test, with different doses of Kangke injection (7.5, 15, and 30 mg/kg). The specific mRNA of the P450 enzyme gene (β-actin/P450). Results The specific mRNA of β-actin/P450 mRNA (P-actin/P450) increased with the dose and duration of anticoagulant injection (P<0.01). Conclusion The ratio of P450 increases with the increase of drug dose and time, which can be regarded as an adaptive response to the body’s resistance to poisoning. It can be used for toxicological studies of related drugs.