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利用江南越桔的茎段为外植体,进行其组培快繁技术体系研究。结果表明,WPS+6-BA 3.0 mg/L+NAA 0.2 mg/L+蔗糖20 g/L为诱导丛生芽较佳的启动培养基;WPS+ZT 1.0 mg/L+6-BA 0.2 mg/L+NAA 0.5 mg/L+蔗糖30 g/L为组培苗叶片诱导不定芽增殖较佳的培养基;1/2MS+IBA 0.2 mg/L+NAA 0.5 mg/L+活性炭3.5 mg/L为丛生芽生根较佳的培养基。
Using the stem segments of southern cowberry as explants, the tissue culture technique was studied. The results showed that WPS + 6-BA 3.0 mg / L + NAA 0.2 mg / L + sucrose 20 g / L was the best starter medium for inducing shoots. WPS + ZT 1.0 mg / L + NAA 0.5 mg / L + sucrose 30 g / L was the best medium for inducing adventitious buds proliferation in the tissue culture seedling leaves; 1 / 2MS + IBA 0.2 mg / L + NAA 0.5 mg / L + Good medium.