论文部分内容阅读
AIM:To evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin(IMN)-induced gastropathy.METHODS:Male Sprague-Dawley rats were randomly divided into three groups.Group 1(control,n=6)was given distilled water(DW)orally.Group 2(IMN,n=6)was given oral IMN(150 mg/kg)dissolved in5%sodium bicarbonate(Na HCO3-)at time 0 and 4 h.Group 3(Aloe vera-treated,n=6)was given oral Aloevera(150 mg/kg)dissolved in DW and IMN at time 0and 4 h.Eight hours later,the stomach was removed to determine gastric malondialdehyde(MDA),the number of interleukin(IL)-18 positive stained cells(%)by immunohistochemistry,and for histopathological examination.Then,the serum was collected to determine tumor necrosis factor(TNF)-αand cytokineinduced neutrophil chemoattractant(CINC)-1 by sandwich enzyme linked immunosorbent assay method.RESULTS:In the IMN group,serum TNF-α,CINC-1and gastric MDA were significantly increased when compared to the control group(27.78±1.52 pg/m L vs 85.07±49.11 pg/m L,P=0.009;104.55±45.80pg/m L vs 1054.70±20.38 pg/m L,and 1.74±0.21nmol/mg vs 9.36±1.07 nmol/mg protein,P=0.000,respectively).The mean level of TNF-α,CINC-1 and gastric MDA in the Aloe vera-treated group were improved as compared with the IMN group(85.07±49.11 pg/m L vs 35.19±1.61 pg/m L,P=0.021;1054.70±20.38 pg/m L vs 813.56±239.04 pg/m L,P=0.025;and 9.36±1.07 nmol/mg vs 2.67±0.64 nmol/mg protein,P=0.000,respectively).The number of IL-18 positive stained cells(%)in the gastric epithelial cells of the IMN group was significantly higher than the control group(5.01%±3.73%vs 30.67%±2.03%,P=0.000,respectively).In contrast,Aloe vera treatment decreased the number of IL-18 positive stained cells(%)significantly when compared with the IMN group(30.67%±2.03%vs 13.21%±1.10%,P=0.000,respectively).Most rats in the IMN group developed moderate to severe gastric inflammation and erosions.The gastric erosions and neutrophil infiltration scores were significantly reduced in the Aloe veratreated group.CONCLUSION:Aloe vera attenuated IMN-induced gastropathy in rats by the reduction of oxidative stress,inflammation,and improvement of gastrichistopathology.
AIM: To evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin (IMN) -induced gastropathy. METHODS: Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control, n = 6) was given distilled Water (DW) orally. Group 2 (IMN, n = 6) was given oral IMN (150 mg / kg) dissolved in 5% sodium bicarbonate (Na HCO3-) at time 0 and 4 h. Group 3 (Aloe vera-treated, n = 6) was given oral Aloevera (150 mg / kg) dissolved in DW and IMN at time 0 and 4 h. Light hours later, the stomach was removed to determine gastric malondialdehyde (MDA), the number of interleukin (IL) positive stained cells (%) by immunohistochemistry, and for histopathological examination. Chen, the serum was collected to determine tumor necrosis factor (TNF) -α and cytokine induced uutrophil chemoattractant (CINC) -1 by sandwich enzyme linked immunosorbent assay method. IMN group, serum TNF-α, CINC-1 and gastric MDA were significantly increased compared to the control group (27.78 ± 1.52 pg / m L vs 85.07 ± 49.1 1 pg / m L, P = 0.009; 104.55 ± 45.80 pg / m L vs 1054.70 ± 20.38 pg / m L, and 1.74 ± 0.21 nmol / mg vs 9.36 ± 1.07 nmol / mg protein, P = 0.000, respectively) mean level of TNF-α, CINC-1 and gastric MDA in the Aloe vera-treated group were improved as compared with the IMN group (85.07 ± 49.11 pg / m L vs 35.19 ± 1.61 pg / m L, P = 0.021; ± 20.38 pg / m L vs. 813.56 ± 239.04 pg / m L, P = 0.025; and 9.36 ± 1.07 nmol / mg vs. 2.67 ± 0.64 nmol / mg protein, P = 0.000, respectively) (%) in the gastric epithelial cells of the IMN group was significantly higher than the control group (5.01% ± 3.73% vs 30.67% ± 2.03%, P = 0.000, respectively) .In contrast, Aloe vera treatment decreased the number of IL-18 positive stained cells (%) significantly when compared with the IMN group (30.67% ± 2.03% vs 13.21% ± 1.10%, P = 0.000, respectively) .Most rats in the IMN group developed moderate to severe gastric inflammation and erosions The gastric erosions and neutrophil infiltration scores were significantly reduced in the A loe veratreated group.CONCLUSION: Aloe vera attenuated IMN-induced gastropathy in rats by the reduction of oxidative stress, inflammation, and improvement of gastrichistopathology.