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目的:研究骨髓中己糖激酶Ⅰ(HKⅠ)在慢性粒细胞白血病(CML)患者由慢性期(CP)向急变期(BP/BC,急髓变)转变过程中表达水平的变化。方法:选择慢性粒细胞白血病患者71例,其中实验组51例(包括24例急变期患者、27例慢性期患者),对照组20例。取实验组和对照组患者骨髓液,RT-PCR法检测HKⅠmRNA;免疫组织化学法检测HKⅠ在组织中的表达水平。用图像分析系统计算IOD值作为mRNA和蛋白表达量。结果:转录表达水平测定,CML急变期患者IOD值为0.836±0.105,慢性期患者为0.418±0.107,对照组为0.122±0.051,急变期患者明显高于慢性期患者,后者高于对照组,P<0.01;蛋白表达水平测定,CML急变期患者为1.230±0.187,慢性期为0.671±0.110,对照组为0.214±0.083,急变期患者明显高于慢性期患者,后者高于对照组,P<0.01。结论:转录表达水平及蛋白表达水平均显示,HKⅠ表达急变期、慢性期、对照组逐渐降低,提示HKⅠ可能参与了CML的发病机制,HKⅠ表达改变可能是促使CML由慢性期向急变期转变的另外一个条件,可联合其他指标一起作为病情监测的指标。
OBJECTIVE: To study the changes of the expression of hexokinase Ⅰ (HKⅠ) in the bone marrow of patients with chronic myeloid leukemia (CML) from chronic phase (CP) to blast crisis (BP / BC, acute myeloid). Methods: A total of 71 patients with chronic myeloid leukemia were selected, including 51 patients in experimental group (including 24 patients with acute phase and 27 patients with chronic phase) and 20 patients in control group. The bone marrow of the patients in the experimental group and the control group were taken and the HKⅠmRNA was detected by RT-PCR. The expression of HKⅠ in the tissue was detected by immunohistochemistry. The image analysis system was used to calculate IOD values as mRNA and protein expression levels. Results: The IOD value was 0.836 ± 0.105 in acute phase of CML, 0.418 ± 0.107 in chronic phase and 0.122 ± 0.051 in control group. The level of transcription was significantly higher in patients with acute phase of CML than that in chronic phase, P <0.01; The level of protein expression in acute phase of CML was 1.230 ± 0.187, in chronic phase was 0.671 ± 0.110, in control group was 0.214 ± 0.083, in acute phase was significantly higher than in chronic phase, the latter was higher than that in control group, P <0.01. CONCLUSION: Both transcriptional expression and protein expression levels show that HK Ⅰ expression is gradually decreased in acute phase and chronic phase, which indicates that HK Ⅰ may be involved in the pathogenesis of CML. The change of HK Ⅰ may lead to the change of CML from chronic phase to blastic phase Another condition, together with other indicators can be used as an indicator of disease surveillance.