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以美洲商陆(Phytolacca americana L.)叶、下胚轴、根尖为外植体,初步研究了3者最适于诱导形成愈伤组织的激素种类和浓度水平.研究证实,叶诱导愈伤组织的最佳培养基为:MS+0.1 mg.L-16-苄基腺嘌呤(6-BA)+0.1 mg.L-1吲哚丁酸(IBA)+0.1 mg.L-1赤霉素(GA)3+300mg.L-1水解乳蛋白(LH)+30g.L-1蔗糖+9.0g.L-1琼脂,pH5.8.下胚轴诱导愈伤组织的最佳培养基为:MS+0.1mg.L-16-苄基腺嘌呤(6-BA)+0.1 mg.L-1赤霉素(GA)3+300 mg.L-1水解乳蛋白(LH)+30 g.L-1蔗糖+9.0 g.L-1琼脂,pH5.8.根尖诱导愈伤组织的最佳培养基为:MS+5.0 mg.L-16-苄基腺嘌呤(6-BA)+5.0 mg.L-1吲哚丁酸(IBA)+0.1 mg.L-1赤霉素(GA3)+300 mg.L-1水解乳蛋白(LH)+30g.L-1蔗糖+9.0g.L-1琼脂,pH5.8.
Using the leaves, hypocotyls and apices of Phytolacca americana L. as the explants, the three most suitable hormones for inducing the formation of callus were studied, and the results showed that leaf-induced callus The optimal culture medium was: MS + 0.1 mg.L-16-benzyladenine (6-BA) +0.1 mg.L -1 indole butyric acid (IBA) +0.1 mg.L -1 gibberellin (GA) 3 + 300mg.L-1 Hydrolyzed milk protein (LH) + 30g.L-1 sucrose + 9.0gL-1 agar, pH5.8.The optimal culture medium for hypocotyl induced callus was MS + 0.1 mg L-16-benzyladenine (6-BA) + 0.1 mg L -1 g GA 3 + 300 mg L -1 lactalbumin LH + 30 g L -1 sucrose + 9.0 gL-1 agar at pH 5.8. The best medium for root induction was MS + 5.0 mg.L-16-benzyladenine (6-BA) +5.0 mg.L-1indole Butyric acid (IBA) +0.1 mg.L-1 GA3 +300 mg.L-1 Hydrolyzed milk protein (LH) +30 g.L-1 sucrose + 9.0 gL-1 agar, pH 5.8.