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目的:探讨绿原酸对缺氧复氧导致的心肌细胞内锚定蛋白-B(ankyrin-B)下降的调节作用及其机制。方法:培养乳鼠心肌细胞,分为对照组、缺氧复氧组和绿原酸处理组。以流式细胞法检测心肌细胞内活性氧(ROS)的水平,用Western-blot检测心肌细胞内ankyrin-B的表达。结果:缺氧复氧后心肌细胞内ROS水平明显增高,绿原酸预处理能够抑制ROS水平的增高[对照组:(237.6±22.3);缺氧复氧组:(612.1±25.6),绿原酸处理组:(375.9±30.1);P<0.05]。缺氧复氧后心肌细胞内ankyrin-B的表达显著降低,绿原酸预处理能够上调ankyrin-B的表达。结论:绿原酸可能通过清除细胞内的ROS从而减少缺氧复氧引起的心肌细胞内ankyrin-B的表达降低。
Objective: To investigate the regulatory effect of chlorogenic acid on the decrease of ankyrin-B in cardiomyocytes induced by hypoxia-reoxygenation and its mechanism. Methods: The neonatal rat cardiomyocytes were cultured and divided into control group, hypoxia-reoxygenation group and chlorogenic acid group. The level of reactive oxygen species (ROS) in cardiomyocytes was detected by flow cytometry. The expression of ankyrin-B in cardiomyocytes was detected by Western-blot. RESULTS: After hypoxia / reoxygenation, ROS level in cardiomyocytes was significantly increased, while chlorogenic acid pretreatment could inhibit the increase of ROS levels in the control group (237.6 ± 22.3), hypoxia-reoxygenation group (612.1 ± 25.6) Acid treatment group: (375.9 ± 30.1); P <0.05]. After anoxia-reoxygenation, the expression of ankyrin-B in cardiomyocytes was significantly decreased, and chlorogenic acid preconditioning could up-regulate the expression of ankyrin-B. CONCLUSION: Chlorogenic acid may reduce the expression of ankyrin-B in cardiomyocytes induced by hypoxia-reoxygenation by clearing ROS in cells.