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用克山病病区粮配成基础低硒饲料,补充硒和/或维生素E组成四种不同水平的饲料,饲喂雄性断乳大鼠,观察其对机体抗氧化能力的影响。评价指标是用抗坏血酸诱发的红细胞溶血率、被O~-_2(超氧阴离子)氧化的血红蛋白量和组织中的TBA值。动物饲养13周后,自尾静脉取血,测定溶血率和血红蛋白被氧化的百分率,和全血SeGSHPx(含硒谷胱甘肽过氧化物酶)活力。15周后将动物断头杀死,立即取出心脏和肝脏测定SeGSHPx活力和TBA值。结果表明在克山病病区粮的饲料中补充硒或维生素E,或者二者同时补充均明显提高组织中的SeGSHPx活力和降低组织中的TBA值。不论在硒缺乏时或硒充足时,饲料中补充维生素E显著降低抗坏血酸诱发的红细胞溶血率,对O~-_2氧化血红蛋白无保护作用。在维生素E缺乏时,仅补充硒对溶血无作用。不论饲料中维生素E缺乏或者充足,补充硒对O~-_1氧化血红蛋白均有显著保护作用。
With Keshan disease ward allocated to the basic low-selenium feed, selenium and / or vitamin E supplemented with four different levels of feed, fed male weanling rats to observe the impact on the body’s antioxidant capacity. The evaluation criteria were erythrocyte hemolysis rate induced by ascorbic acid, amount of hemoglobin oxidized by O-2 (superoxide anion), and TBA value in the tissue. Thirteen weeks after the animals were bled, blood was drawn from the tail vein to measure the rate of hemolysis and the percentage of hemoglobin that was oxidized, and whole blood SeGSHPx (selenium-containing glutathione peroxidase) activity. After 15 weeks the animals were decapitated and the heart and liver removed immediately for measurement of SeGSHPx activity and TBA values. The results showed that supplementation of dietary supplements with selenium or vitamin E in Keshan disease wards or both simultaneously significantly increased the activity of SeGSHPx in tissues and decreased the TBA in tissues. In the absence of selenium or adequate selenium, the dietary vitamin E supplementation significantly reduced ascorbate-induced erythrocyte hemolysis and had no protective effect on O ~ -oxidized hemoglobin. In the absence of vitamin E, selenium supplementation alone has no effect on hemolysis. Regardless of dietary vitamin E deficiency or adequate, selenium supplementation on O ~ -1 oxyhemoglobin has a significant protective effect.