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目的探讨如何建立稳定的小鼠颈部心脏移植模型。方法将40只雄性C57小鼠,周龄约6~8周,体质量19~24 g,随机分成供体和受体,每组20只。供体心脏升主动脉与受体右侧颈总动脉端侧吻合,供体心脏肺动脉与受体右侧颈外静脉对端吻合,建立颈部心脏移植模型。结果手术成功率在95%以上,冷缺血时间(15±5)min,热缺血时间(23±6)min,手术时间(55±15)min,术后移植心脏存活达到30 d以上,术后1个月移植心脏病理检查和正常心脏无明显差异。结论小鼠颈部心脏移植模型稳定可靠,通过直视和触诊可以方便监测供体心脏的存活,有利于开展移植方面的基础研究。
Objective To explore how to establish a stable model of cervical heart transplantation in mice. Methods Forty male C57 mice aged 6-8 weeks and 19-24 g were randomly divided into donor and recipient groups, 20 in each. The ascending aorta of the donor heart was anastomosed to the right common carotid artery and the donor’s pulmonary artery was anastomosed to the right external jugular vein of the recipient to establish a cervical heart transplant model. Results The success rate of operation was above 95%, the duration of cold ischemia (15 ± 5) min, warm ischemia (23 ± 6) min, operation time (55 ± 15) min, postoperative graft survival more than 30 d, One month after transplantation, there was no significant difference in cardiac pathology and normal heart. Conclusion The model of cervical heart transplantation in mice is stable and reliable. It is easy to monitor donor heart survival by direct vision and palpation, which is conducive to the basic research of transplantation.