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目的研究海洋细菌成团泛菌(Pantoea agglomerans)发酵液的乙酸乙酯萃取物中的化学成分,以期得到有活性的先导化合物。方法采用硅胶柱色谱、凝胶柱色谱、高效液相色谱等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构。结果从海洋细菌Pantoea agglomer-ans的乙酸乙酯萃取物中分离得到10个环二肽类化合物,分别鉴定为环-(苯丙氨酸-脯氨酸)[cy-clo(Phe-Pro),1]、环-(缬氨酸-脯氨酸)[cyclo(Val-Pro),2]、环-(丙氨酸-亮氨酸)[cyclo(Ala-Leu),3]、环-(色氨酸-脯氨酸)[cyclo(Trp-Pro),4]、环-(色氨酸-甘氨酸)[cyclo(Trp-Gly),5]、环(4-羟基-脯氨酸-苯丙氨酸)[cyclo(4-hydroxyl-Pro-Phe),6]、环-(脯氨酸-酪氨酸)[cyclo(Pro-Tyr),7]、环-(亮氨酸-亮氨酸)[cyclo(Leu-Leu),8]、环-(脯氨酸-亮氨酸)[cyclo(Pro-Leu),9]、环-(缬氨酸-异亮氨酸)[cyclo(Val-Ile),10]。结论化合物1~10均为首次从海洋细菌Pantoea agglomerans中分离得到。
Objective To study the chemical constituents of the ethyl acetate extracts of marine bacteria Pantoea agglomerans fermentation broth in order to obtain active lead compounds. Methods The compounds were separated and purified by silica gel column chromatography, gel column chromatography and high performance liquid chromatography. The structures of the compounds were identified by physicochemical properties and spectral data analysis. Results Ten cyclic dipeptides were isolated from the ethyl acetate extracts of marine bacteria Pantoea agglomer-ans and identified as cy-clo (Phe-Pro), 1], Cyclo- (Val-Pro), Cyclo- (alanine-Leu), Cyclo- Tryptophan-proline) [cyclo (Trp-Pro), 4], cyclo- (tryptophan- glycine) Cyclo [(Pro-Tyr), 7], cyclo- (leucine-leucine) [Cyclo (Leu-Leu), 8], cyclo- (proline-leucine) [cyclo Val-Ile), 10]. Conclusion Compounds 1-10 were isolated from marine bacteria Pantoea agglomerans for the first time.