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Objective:To investigate the role of Mdm2 expression in hepatocellular carcinoma.Methods: Streptavidin-peroxidase conjugation method (SP)was used to observe the expression of Mdm2 and p53 proteins in 61 cases of hepatocellular carcinoma(HCC)and 59 cases of corresponding paracancerous tissues,among which p53 mutations in exons 5～8 were detected in 21 cases by PCR-SSCP.Results:Positive nuclear P53 and Mdm2 immunostains were demonstrated in 57.38% and 26.23% of HCC,1.69% and 3.39% of corresponding paracancerous tissues respectively.The expression of p53 and Mdm2 proteins in hepatocellular carcinoma was significantly higher than that in paracancerous tissues( P <0.01).The expression of P53 and Mdm2 was not significantly correlated.42.86% of hepatocellualr carcinomas showed mutations in exon 7 of p53 gene,and no mutation was found in exons 5,6,8 and paracancerous tissues. 66.67% of mutational cases had P53 overexpression and 11.11%(1/9)showed overexpression of both P53 and Mdm2,Mdm2 overexpression also appeared in 25% of cases without mutations.Conclusions:Mdm2-induced p53 inactivation and p53 gene mutation play an important role in carcinogenesis of hepatocellular carcinoma.Tumorigenic property of Mdm2 itself,together with p53 mutation,may take part in hepatocarcinogenesis.
Objective: To investigate the role of Mdm2 expression in hepatocellular carcinoma.Methods: Streptavidin-peroxidase conjugation method (SP) was used to observe the expression of Mdm2 and p53 proteins in 61 cases of hepatocellular carcinoma (HCC) and 59 cases of corresponding paracancerous tissues ,among which p53 mutations in exons 5～8 were detected in 21 cases by PCR-SSCP.Results:Positive nuclear P53 and Mdm2 immunostains were demonstrated in 57.38% and 26.23% of HCC,1.69% and 3.39% of corresponding paracancerous tissues respectively. The expression of p53 and Mdm2 proteins in hepatocellular carcinoma was significantly higher than that in paracancerous tissues ( P <0.01). The expression of P53 and Mdm2 was not significantly correlated.42.86% of hepatocellualr carcinomas showed mutations in exon 7 of p53 gene,and 66.67% of mutational cases had P53 overexpression and 11.11%(1/9)showed overexpression of both P53 and Mdm2,Mdm2 ove Rexpression also appeared in 25% of cases without mutations.Conclusions:Mdm2-induced p53 inactivation and p53 gene mutation play an important role in carcinogenesis of hepatocellular carcinoma.Tumorigenic property of Mdm2 itself,together with p53 mutation,may take part in hepatocarcinogenesis.