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背景:神经轴突导向因子1作为血管内皮细胞的促有丝分裂原,能够促进血管内皮细胞的迁移和增殖,发挥诱导血管新生的作用。目的:观察神经轴突导向因子1裸质粒转染对5/6肾切除大鼠模型残余肾功能的保护及对肾小管周围毛细血管网的影响。方法:将30只SD大鼠随机等分为假手术组、模型组和治疗组。模型组和治疗组大鼠切除左侧肾脏上、下极各1/3,1周后切除右肾,建立残肾模型。模型组和治疗组大鼠于切除右肾的同时在左肾静脉分别注射空质粒IRES2-EGFP和pC MV6-XL5-Netrin-1-IRES2-EGFP。结果与结论:与模型组相比,治疗组大鼠血尿素氮、血肌酐水平降低,肾间质纤维化程度减轻,肾小管周围毛细血管网的密度增加,肾小管胞浆中神经轴突导向因子1蛋白表达增加。提示神经轴突导向因子1裸质粒转染能明显改善5/6肾切除模型大鼠的肾功能,减轻残肾组织的病理损害和肾间质纤维化面积,增加肾小管周围毛细血管网密度、降低缺氧诱导因子1α表达,从而改善肾间质小管缺氧状态。
BACKGROUND: Axonal targeting factor 1, as a mitogen of vascular endothelial cells, promotes the migration and proliferation of vascular endothelial cells and exerts the effect of inducing angiogenesis. OBJECTIVE: To observe the effect of naked plasmid transfected with axon guiding factor 1 on the remnant renal function in 5/6 nephrectomized rats and on the renal capillary network. Methods: Thirty SD rats were randomly divided into sham operation group, model group and treatment group. In the model group and the treatment group, the right kidney was excised from the upper and lower pole of the left kidney by one-third and one-week respectively, and the remnant kidney model was established. The model group and the treatment group rats were injected with empty plasmid IRES2-EGFP and pC MV6-XL5-Netrin-1-IRES2-EGFP in the left renal vein while excising the right kidney. RESULTS AND CONCLUSION: Compared with model group, the levels of blood urea nitrogen, serum creatinine, the degree of renal interstitial fibrosis, the density of capillary network around the renal tubule increased, the level of neuronal axon in renal tubular cytoplasm Factor 1 protein expression increases. It is suggested that the naked plasmid transfected with axon guiding factor 1 can significantly improve renal function in 5/6 nephrectomized rats, reduce pathological damage and area of renal interstitial fibrosis, increase the capillary network density around the renal tubules, Reduce hypoxia inducible factor 1α expression, thereby improving renal interstitial hypoxia.