固紫染色法(革兰氏染色法),就是用来初步鉴别细菌种属的一种最常用的染色法。它是先将结晶紫、草酸铵染液,加于已加热固定的标本上,染色一分钟,用水冲洗后,再加媒染剂复方碘液(革兰氏碘液,淡碘液)染色一分钟,用水冲洗后,用95％酒精脱色至不见紫色脱落为止(约需一分钟),再用水冲洗,最后用沙黄液或稀石碳 Solid purple staining (Gram staining), is used to initially identify bacterial species is the most commonly used staining. It is the first crystal violet, ammonium oxalate dye solution was added to the heated fixed specimens, staining for one minute, rinsed with water, then add mordant compound iodine solution (Gram iodine solution, light iodine solution) for one minute , Washed with water, decolorized with 95% alcohol to see purple off (about one minute), then rinse with water, and finally with the yellow liquid or thin carbon