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目的 构建一种能在卵巢癌肿瘤组织中进行肿瘤特异性表达的逆转录病毒载体 ,增强肿瘤基因治疗的靶向性。方法 PCR法获得 HER2基因的启动子片段并将其克隆至逆转录病毒载体 PL XSN中 ,再将突变型绿色荧光蛋白 (mt GFP)基因克隆至该启动子下游 ,将此载体转染 SK- OV3细胞和 MCF- 7细胞。结果 转染 3天后可见部分 SK- OV3细胞呈现绿色荧光 ,而 MCF- 7中无此现象。结论 HER- 2基因启动子可控制报告基因在卵巢癌细胞系中进行高效特异性表达 ,此载体经修饰后可用作卵巢癌肿瘤特异性基因治疗的研究工具
Objective To construct a retroviral vector capable of tumor-specific expression in ovarian cancer tissues and to enhance the targeting of tumor gene therapy. Methods The promoter fragment of HER2 gene was obtained by PCR and cloned into the retrovirus vector PL XSN. The mutant green fluorescent protein (mt GFP) gene was cloned downstream of the promoter. The vector was transfected into SK-OV3 Cells and MCF-7 cells. Results Some SK-OV3 cells showed green fluorescence after 3 days of transfection, but no such phenomenon in MCF-7. Conclusion The HER-2 gene promoter can control the expression of the reporter gene in ovarian cancer cell lines with high specificity. The vector can be used as a research tool for ovarian cancer-specific gene therapy