论文部分内容阅读
野生型p53蛋白对细胞增生有负调节作用,因此野生型p53基因是一种抑癌基因。有认为p53基因突变后,不仅失去原有抑癌基因的作用,有时甚至还具有致癌基因的作用。 美国Johns Hopkins大学肿瘤中心曾将野生型p53基因及突变型p53基因（Codon 143 VAL TOALA）分别转染到原来不具有p53基因的髓性白血病HL60细胞株内,使后者具有表达野生型或突变型p53的功能。本实验比较这三种细胞（以下简称野生型,突变型和母系）的生长速率及它们在实验鼠
Wild-type p53 protein negatively regulates cell proliferation, so the wild-type p53 gene is a tumor suppressor gene. It is believed that after mutation of p53 gene, it not only loses the role of the original tumor suppressor gene, but also sometimes has the role of an oncogene. At the Cancer Center of Johns Hopkins University in the US, the wild-type p53 gene and the mutant p53 gene (Codon 143 VAL TOALA) were transfected into the myeloid leukemia HL60 cell line that did not originally harbor the p53 gene, resulting in the expression of wild-type or mutants. P53 function. This experiment compares the growth rates of these three types of cells (herein referred to as wild-type, mutant, and maternal) and their use in experimental mice.