论文部分内容阅读
目的探讨CXCR7-shRNA慢病毒表达载体靶向抑制CXCR7的表达对人结肠癌细胞增殖、迁移活性的影响。方法将人结肠癌细胞HT-29分为实验组、阴性对照组和空白对照组;实验组加入CXCR7-shRNA慢病毒表达载体;阴性对照组加入CXCR7-shRNA慢病毒阴性对照;空白对照组不做任何处理。W estrn b lot方法测定各组细胞CXCR7蛋白表达水平。细胞活性实验、Transwell细胞迁移实验检测各组细胞的增殖和迁移活力。结果实验组CXCR7 mRNA相对表达水平低于阴性对照组和空白对照组(P均<0.05);细胞活性实验显示实验组细胞生长曲线较平缓,细胞生长速度较阴性对照组和空白对照组显著降低(P均<0.05);Transwell细胞迁移实验显示实验组穿透滤过膜细胞数量较阴性对照组和空白对照组明显减少(P均<0.05)。结论靶向沉默CXCR7的表达抑制结肠癌细胞增殖和迁移活性。
Objective To investigate the effect of CXCR7-shRNA lentivirus expression vector targeting CXCR7 on the proliferation and migration of human colon cancer cells. Methods HT-29 human colon cancer cells were divided into experimental group, negative control group and blank control group; CXCR7-shRNA lentivirus expression vector was added into experimental group; CXCR7-shRNA lentivirus negative control group was added into negative control group; blank control group was not made Any deal. W estrn b lot method to determine the expression of CXCR7 protein in each group of cells. The cell viability assay and Transwell cell migration assay were used to detect the proliferation and migration of cells in each group. Results The relative expression level of CXCR7 mRNA in the experimental group was lower than that in the negative control group and blank control group (all P <0.05). The cell viability assay showed that the cell growth curve of the experimental group was gentle and the cell growth rate was significantly lower than that of the negative control group and the blank control group P <0.05). Transwell cell migration assay showed that the number of penetrating membrane cells in experimental group was significantly decreased compared with that of negative control group and blank control group (all P <0.05). Conclusion The expression of targeted silencing CXCR7 inhibits the proliferation and migration of colon cancer cells.