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目的:初步探讨芦荟促进大鼠多柔比星外渗性损伤创面愈合的机制。方法:将45只多柔比星外渗性损伤模型大鼠随机分为生理盐水组(1mL/kg)、硫酸镁组(1mL/kg)和芦荟组(2.5g/kg),每组15只,各组用药后第5天切除外渗性损伤部位的皮肤及皮下组织,间断缝合,术后第7天拆线,观察手术伤口Ⅰ期愈合情况和愈合时间。免疫组化法检测损伤部位的皮肤及皮下组织中的CD105、细胞黏附分子-1(ICAM-1)和血管内皮生长因子(VEGF)的表达情况。结果:三组大鼠的手术伤口Ⅰ期愈合率分别为33.3%、73.3%和86.7%,伤口愈合时间分别为(24.5±4.9)、(15.8±3.6)和(14.0±3.5)d,生理盐水组与另外两组之间,差异均有统计学意义,P<0.05。免疫组化结果显示,芦荟组与生理盐水组比较,VEGF、CD105的表达呈现上调,而ICAM-1的表达呈现下调。结论:芦荟能够显著促进外渗性伤口愈合,其作用机制可能与其促进VEGF、CD105的表达和抑制ICAM-1的表达有关。
OBJECTIVE: To investigate the mechanism of Aloe vera on wound healing induced by extravasation of doxorubicin in rats. Methods: Forty-five adriamycin-induced extravasation injury rats were randomly divided into normal saline group (1mL / kg), magnesium sulfate group (1mL / kg) and aloe vera group (2.5g / kg) The skin and subcutaneous tissue at the site of extravasation injury were excised on the fifth day after the administration. The stitches were sutured intermittently. The stitches were removed on the 7th day after operation. The healing status and the healing time of the wounds were observed. The expression of CD105, ICAM-1 and VEGF in the skin and subcutaneous tissue were detected by immunohistochemistry. Results: The rate of primary wound healing in the three groups was 33.3%, 73.3% and 86.7% respectively, and the wound healing time was (24.5 ± 4.9), (15.8 ± 3.6) and (14.0 ± 3.5) d, The difference between the two groups was statistically significant (P <0.05). The results of immunohistochemistry showed that the expressions of VEGF and CD105 were up-regulated in aloe group compared with saline group, while the expression of ICAM-1 was down-regulated. Conclusion: Aloe vera can significantly promote the healing of extravasated wounds. The mechanism may be related to the promotion of the expression of VEGF and CD105 and the inhibition of ICAM-1 expression.