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目的:探讨唐古特大黄多糖组分1(RTP1)对急性电离辐射损伤小鼠的保护作用。方法:采用昆明种小鼠,随机分为5组:正常对照组(Normal Control,NC)、辐射对照组(Irradiation Control,IC)以及RTP1低剂量组(200 mg/kg)、中(400 mg/kg)和高剂量组(800 mg/kg),采用灌胃给药方式,连续14 d,NC组和IC组则给予等量的生理盐水,第14 d除NC组外,各组小鼠均接受2.0 Gy/只60Coγ射线照射1次,照射后24 h,检测小鼠胸腺和脾脏指数,测定肝脏超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性及丙二酰二醛(MDA)水平以及小鼠外周血象和骨髓嗜多染红细胞(PCE)微核数。结果:RTP1能够升高小鼠的胸腺、脾脏指数,增加肝脏SOD和GSH-Px活性,降低MDA水平,升高外周血中白细胞数并降低骨髓PCE微核数,与IC组比较有统计学意义(P<0.05或P<0.01)。结论:RTP1对辐射所致的小鼠损伤具有一定的保护作用。
Objective: To investigate the protective effect of Ruthenium tiglicum polysaccharide fraction 1 (RTP1) on acute ionizing radiation-induced injury in mice. Methods: Kunming mice were randomly divided into 5 groups: Normal Control (NC), Irradiation Control (IC) and RTP1 low dose (200 mg / kg) kg) and high dose group (800 mg / kg) for 14 days. The rats in NC group and IC group were given the same amount of saline. On the 14th day, The mice were subjected to 2.0 Gy / 60Co γ-ray irradiation for 1 hour and the thymus and spleen indexes of mice were measured 24 h after irradiation. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) Malondialdehyde (MDA) levels and micronuclei in peripheral blood and bone marrow polychromatic erythrocytes (PCE) in mice. Results: RTP1 increased the thymus and spleen index, increased the activity of SOD and GSH-Px in liver, decreased the level of MDA, increased the number of white blood cells in peripheral blood and decreased the number of micronuclei in PCE of bone marrow, which was statistically significant compared with IC group (P <0.05 or P <0.01). Conclusion: RTP1 has certain protective effect on radiation-induced mouse injury.