为比较有创与无创冷冻方法处理的肌腱异体移植的效果，探索不同冷冻处理技术对肌腱免疫源性的影响及获得成功的异体移植的条件，采用有创反复冻融及无创超深低温（－１９６℃）冻存技术分别处理肌腱，液氮下保存３个月后异体移植，以自体肌腱移植作对照。腱细胞活力测定显示，反复冻融处理的肌腱细胞全部失活，而超深低温处理的肌腱细胞活力为（９２．５±３．４）％。组织学观察显示，反复冻融及超深低温处理的异体移植肌腱均产生了不同程度炎性细胞浸润，且腱周粘连均较自体移植肌腱重。主动屈曲功能测定、羟脯氨酸含量测定及生物力学性能测定显示，反复冻融与超深低温组无显著性差异（Ｐ＞０．０５），且后两项指标显著较自体移植肌腱差。认为：①反复冻融及超深低温处理的肌腱异体移植获得一定成功，两者无显著性意义。②既要保留肌腱细胞的活性，又要去除肌腱中的抗原呈递细胞，可能是冷冻处理的肌腱异体移植获得成功的关键。③损伤了肌腱细胞成分，降低了肌腱抗原性，不等于能获得成功的异体移植 In order to compare the effect of the tendon allotransplantation treated by invasive and noninvasive freezing methods and explore the effect of different cryogenic treatment on the immunogenicity of tendon and the conditions of successful allotransplantation, 196 ℃) cryopreservation techniques were treated tendon, three months after storage in liquid nitrogen allograft, with autologous tendon transplantation as a control. Tendon cell viability assay showed that all of the tendon cells in the freeze-thaw cycles were completely inactivated while the viability of the tendon cells in ultra-deep hypothermia treatment was (92.5 ± 3.4)%. Histological observation showed that repeated freeze-thaw and ultra-deep cryogenic treatment of allograft tendon have different degrees of inflammatory cell infiltration, and the tendon adhesion than autologous tendon grafts. Determination of active flexion, determination of hydroxyproline content and biomechanical properties showed no significant difference between repeated freeze-thawing and ultra-deep hypothermia (P> 0.05), and the latter two indexes were significantly lower than those of autograft tendons. It is concluded that: ①There are some success of tendon allograft after repeated freezing and thawing and ultra-deep hypothermia, both of which have no significant significance. ② It is necessary to retain the activity of tendon cells, but also remove the antigen-presenting cells tendon, frozen tendon allograft may be the key to success. ③ damaged tendon cell components, reducing the tendon antigenicity, does not mean that successful allogeneic transplantation