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近年来,应用松毛虫质型多角体病毒防治松毛虫已成为综合防治的重要手段之一,但要在生产中大面积应用,尚需解决病毒的增殖与提取方法问题。1986—1987年,我们在进行松毛虫质型多角体增殖与提取试验中,总结出塑料薄膜罩围式增殖CPV 和直接从病死虫尸中提取CPV 的新方法。一、试验材料与方法1.病毒:松毛中CPV 毒源系中山大学昆虫所和本所病毒与生防组提供。
In recent years, the application of dendrolimus polyhedrosis virus dendrolimus has become an important means of comprehensive prevention and control, but in the production of large area applications, still need to solve the problem of virus proliferation and extraction methods. In 1986-1987, we conducted a new test on the proliferation and extraction of polyhedra of Dendrolimus sinensis and summarized the plastic membrane enclosure proliferation CPV and the direct extraction of CPV from diseased dead bodies. First, the test materials and methods 1. Virus: CPV virulent hair in the Sun Yat-sen University Institute of Entomology and Entomology and Entomology provided.