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目的研究山茱萸多糖调节小鼠肠道菌群失调的作用。方法按50 mL·kg-1ig给予小鼠0.3 g·mL-1盐酸林可霉素,一天2次,连续3 d,建立小鼠肠道菌群失调模型后,按50 mL·kg-1ig给予小鼠山茱萸多糖(3、2、1 g·mL-1)治疗,连续6 d。同时设正常对照组、阳性对照组、自然恢复组,于实验第10天取新鲜粪便检测肠道菌群(肠杆菌、肠球菌、双歧杆菌和乳杆菌)的变化。结果山茱萸多糖无明显毒性,ig给予小鼠的最大耐受量为150 g·kg-1;山茱萸多糖低、中剂量组小鼠肠道的有益菌双歧杆菌和乳杆菌数量明显上升;中剂量组与正常对照组及自然恢复组比较,差异显著(P<0.05)。结论山茱萸多糖能扶植肠道正常菌群的生长,促进有益菌(双歧杆菌和乳杆菌)的增殖,具有调节肠道菌群失调的作用。
Objective To study the effect of Fructus Corni polysaccharide on the regulation of intestinal microflora in mice. Methods Mice were treated with 0.3 g · mL-1 lincomycin at 50 mL · kg-1, twice a day for 3 days. The mouse model of intestinal flora was established and then treated with 50 mL · kg-1 The mice were treated with dogwood polysaccharide (3, 2, 1 g · mL-1) for 6 days. At the same time, the normal control group, positive control group and natural recovery group were set up. Fresh feces were collected on the 10th day of the experiment to detect the change of intestinal flora (Enterobacteriaceae, Enterococcus, Bifidobacterium and Lactobacillus). Results Fructus Corni polysaccharides no significant toxicity, the maximum tolerated dose of ig given mice was 150 g · kg-1; dogwood polysaccharide low and medium doses of the intestinal tract of beneficial bacteria Bifidobacterium and Lactobacillus significantly increased the number; medium dose Compared with normal control group and natural recovery group, the difference was significant (P <0.05). Conclusion Fructus Corni polysaccharides can support the growth of normal intestinal flora and promote the proliferation of beneficial bacteria (Bifidobacterium and Lactobacillus), which can regulate the imbalance of intestinal flora.