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目的:探讨蜕膜巨噬细胞内活性氧(ROS)、吞噬活性、细胞因子IL-10和IFNγ的分泌,与原因不明复发性流产发病的关系。方法:应用磁珠细胞分选法从11例原因不明复发性流产患者和12例正常早孕妇女蜕膜中分离巨噬细胞,采用流式细胞技术检测蜕膜巨噬细胞内ROS水平,中性红实验检测巨噬细胞吞噬活性,应用ELISPOT法检测细胞因子IL-10和IFNγ分泌。结果:与正常早孕者相比,原因不明复发性流产患者蜕膜巨噬细胞内ROS水平升高(222.55±46.74vs170.75±17.51,P<0.01),其吞噬活性增强(0.37±0.14vs0.17±0.98,P<0.01),IL-10/IFNγ下降(1.37±0.88vs7.69±3.78,P<0.01)。结论:蜕膜巨噬细胞活性改变以及其分泌的细胞因子格局变化可能是导致原因不明复发性流产发病的机制之一。
Objective: To investigate the relationship between reactive oxygen species (ROS), phagocytic activity, secretion of cytokines IL-10 and IFNγ in decidual macrophages and the pathogenesis of unexplained recurrent spontaneous abortion. METHODS: Macrophages were isolated from decidua of 11 patients with unexplained recurrent spontaneous abortion and 12 normal pregnant women by magnetic bead sorting. Flow cytometry was used to detect ROS levels in decidual macrophages. Neutral red The phagocytic activity of macrophages was detected by experiments. The secretion of IL-10 and IFNγ by cytokines was detected by ELISPOT. Results: Compared with normal pregnant women, the levels of ROS in decidual macrophages in patients with unexplained recurrent spontaneous abortion increased (222.55 ± 46.74 vs 170.75 ± 17.51, P <0.01), and their phagocytic activity increased (0.37 ± 0.14 vs 0. 17 ± 0.98, P <0.01), IL-10 / IFNγ decreased (1.37 ± 0.88vs7.69 ± 3.78, P <0.01). CONCLUSION: The changes of the activity of decidual macrophages and the changes of cytokines secreted by them may be one of the mechanisms leading to the pathogenesis of recurrent spontaneous abortion.