作者观察到将氟和铅离子加入白细胞培养液中,中期染色体有明显的改变。血液取自波兰什切青(szczecin)医学科学院的男性供血者,白细胞作96小时的培养,在培养72小时后,加入氟和铅离子,然后细胞经低渗处理、甲醇固定、染色体染色、照相分析、按标准方法对中期畸变进行分类。氟离子(氟化钠)的浓度是0.6、6.0、60 ppm,铅离子(醋酸铅)浓度为2.1和210 ppm,对照组除了不含氟和铅盐外,其他处理均相同。所观察的染色体畸变包括结构异常,如染 The authors observed that fluoride and lead ions were added to the white blood cell culture medium, with significant changes in the metaphase chromosome. Blood was collected from male donors in the Academy of Medical Sciences of Szczecin, Poland, and white blood cells were cultured for 96 hours. Fluorine and lead ions were added after 72 hours of culture, and the cells were subjected to hypotonic treatment, methanol fixation, chromosome staining, Analysis, according to standard methods of classification of medium-term distortions. The concentrations of fluoride (sodium fluoride) were 0.6, 6.0, 60 ppm, and lead (lead acetate) concentrations were 2.1 and 210 ppm, respectively. The control groups were the same except for the fluorine and lead salts. Chromosomal aberrations observed include structural abnormalities such as staining