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通过柑桔五种叶片同工酶的聚丙烯酰胺凝胶电泳分析来探讨柑桔裁培种与野生种的系统发育关系。共分析了谷氨酸草酰乙酸转氨酶(GOT)三个位点、过氧化物歧化酶(SOD)二个位点及苹果酸脱氢酶(MDH)、过氧化物酶(PX)、多酚氧化酶(PPO)的一个位点。 分析结果表明宽皮柑桔类品种有三个遗传起源地,即印度,中国和日本。中国的许多宽皮柑桔类品种[包括乳桔(kinokuna)及朱砂桔(Chu Sha Chu)]具有独特的基因型,而栟柑及丹西蜜桔在PX位点上表现的基因型不同于中国宽皮柑桔类品种,由此表明他们象以前认为的一样起源于印度。另外,被认为起源中国的Szu—ui—Kom则具有一个与印度宽皮柑桔类相同的基因C。
Phylogenetic relationships among cuttings of citrus and wild species were explored by polyacrylamide gel electrophoresis analysis of five leaf isozymes of citrus. Three sites of glutamic oxaloacetic transaminase (GOT), two sites of superoxide dismutase (SOD) and two sites of malate dehydrogenase (MDH), peroxidase (PX), polyphenols One site of oxidase (PPO). The analysis showed that there are three genetic origins of the broad-leaved citrus varieties, namely India, China and Japan. Many Chinese broad-leaved citrus cultivars (including kinokuna and Chu Sha Chu) have unique genotypes, while P. kanshill and Danish tangerine exhibit different genotypes at PX sites Chinese broad-leaved citrus varieties, thus suggesting that they originated in India as they once thought. In addition, Szu-ui-Kom, believed to originate in China, has the same gene C as the broad-leaved citrus in India.