论文部分内容阅读
Objective:To investigate the frequencies of -1470,-511 and -31 single nucleotide polymorphisms (SNPs) in the promoter of IL-1β and its haplotype constitution in Chongqing population.Methods: One hundred and twelve healthy Chongqing people were enrolled in this study.Polymorphisms at -1470 (G to C), -511 (T to C) and -31(C to T ) of IL-1β were genotyped with the method of restriction fragment length polymorphism (RFLP).Haplotype frequencies were analyzed by Arlequine software.Results: Frequencies of IL-1β -1470, -511 and -31 SNPs were 41.67%, 50% and 45.33%, respectively.Genotype frequencies of -1470 locus were 39.81%, 37.04 %and 23.15% for G/G, G/C and C/C respectively. As for T-511C SNP, genotype frequencies of T/T, T/C and C/C were 29.91%, 40.18% and 29.91%, respectively.Genotyping results of C/C, C/T, and T/T of -31 locus were 35.51%, 38.32% and 26.71% respectively.Haplotype analysis found that there were mainly three haplotypes constituted by three SNPs, ie. , G-T-C, C-T-C and G-C-T.Conclusions: Polymorphisms exist in the promoter of IL-1β in Chongqing population. Three SNPs locate in the same haplotype block.