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目的研究肝细胞再生因子对肝细胞增殖的影响。方法利用分子筛层析、高效液相离子交换层析和毛细管电泳从人胎肝中分离肝细胞再生因子，采用［３Ｈ］ＴｄＲ参入法观察其对肝源性细胞的增殖作用。结果肝细胞再生因子显著促进［３Ｈ］ＴｄＲ的参入。结论采用本方法提纯的肝细胞再生因子能有效地促进肝源性细胞增殖。
Objective To study the effect of hepatocyte regeneration factor on hepatocyte proliferation. Methods Hepatocyte regeneration factor was isolated from human fetal liver by molecular sieve chromatography, high performance liquid chromatography (HPLC) and capillary electrophoresis. [3H] TdR incorporation was used to observe the proliferation of hepatocyte. Results Hepatocyte regeneration factor significantly promoted the incorporation of [3H] TdR. Conclusion The hepatocyte regeneration factor purified by this method can effectively promote the proliferation of liver-derived cells.