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为揭示水杨酸(SA)诱导山茶产生抗病性反应机制,采用琼脂平板法测定了SA对山茶灰斑病菌Pestalotiopsis guepinii(Desm.)Stey的影响。结果表明,浓度为0~5mmol/L的SA对该菌的生长没有抑制作用。用SA喷雾涂布叶片诱导山茶抗性,其植株内过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)等防御酶对山茶灰斑病菌诱导信号有不同响应。诱导并挑战接种处理的植株体内上述酶活性比只诱导不接种处理上升速度快,不同浓度的SA诱导及诱导后挑战接种植株体内的POD、PPO、CAT、PAL活性与SA浓度呈正相关。各防御酶活性与感病指数的相关性分析表明,CAT活性与感病指数显著负相关(r=-0.9730),除PPO与感病指数的相关系数很低外,其它酶均较高,尽管未达到显著水平,但仍说明POD、PAL在诱导山茶抗病性中具有重要作用。
To reveal the mechanism of resistance of camellia sinensis (SA) induced by salicylic acid (SA), the effect of SA on Pestalotiopsis guepinii (Desm.) Stey was determined by agar plate method. The results showed that the concentration of 0 ~ 5mmol / L of SA on the growth of the bacteria did not inhibit. The leaves of Camellia japonica L. were sprayed with SA spray to induce the resistance of Camellia sinensis, and the defense of peroxidase (POD), polyphenol oxidase (PPO), catalase (CAT) and phenylalanine ammonia lyase (PAL) The enzyme has a different response to the signal induced by the gray spot scab. The above-mentioned enzyme activity induced and challenged the inoculated plants in vivo was higher than that of the non-inoculated plants alone. The activity of POD, PPO, CAT and PAL in challenge challenged plants were positively correlated with the concentration of SA. The correlation analysis of each defense enzyme activity and susceptibility index showed that there was a significant negative correlation between CAT activity and susceptibility index (r = -0.9730), except that the correlation coefficient between PPO and susceptibility index was very low Did not reach significant level, but still shows POD, PAL in the resistance induced by Camellia plays an important role.