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调节致癌蛋白E6/E7异常表达在人乳头状瘤病毒的致癌过程中具有一定作用。HPV E6/E7 mRNA原位杂交(RISH)检测包括18种常见高危类型(HR-RISH/HR-HPV RNA 18 ISH),在肛门与生殖器肿瘤中尚未得到广泛的研究。作者对HPV相关肛门与生殖器及头颈部肿瘤中,PCR、P16免疫组化染色和HPV DNA原位杂交检测结果与HR-RISH结果进行比较。共搜集102例病理结果异常病例,其中宫颈鳞状上皮内病变(16 CIN1,25 CIN3,3 AIN1,12 AIN3,9 VIN3)及浸润性鳞状细胞癌(17宫颈癌,2外阴肿瘤,18头颈部肿瘤)及10例病理结果为正常宫颈和15例宫颈慢性炎症(对照组)。对福尔马林固定的组织石蜡切片均进行HR-RISH、DNA原位杂交、p16染色及6种低危型HPV RNA原位杂交(LR-RISH)检测,其中RNA和
Regulating the aberrant expression of the oncogenic protein E6 / E7 has a role in the carcinogenesis of human papillomavirus. HPV E6 / E7 mRNA in situ hybridization (RISH) detection, including 18 common high-risk types (HR-RISH / HR-HPV RNA 18 ISH), has not been extensively studied in anal and genital tumors. The authors compared HPV-related anal and genital head and neck tumors with PCR, P16 immunohistochemistry and HPV DNA in situ hybridization and HR-RISH results. A total of 102 cases of abnormal pathological results were collected, including cervical squamous intraepithelial lesions (16 CIN1,25 CIN3,3 AIN1,12 AIN3,9 VIN3) and invasive squamous cell carcinoma (17 cervical cancer, 2 vulvar tumors, 18 Neck tumor) and 10 cases of pathological results for the normal cervix and 15 cases of chronic cervical inflammation (control group). HR-RISH, DNA in situ hybridization, p16 staining and six kinds of low-risk HPV RNA in situ hybridization (LR-RISH) were performed on formalin-fixed tissue paraffin sections. The RNA and