目的探讨miR-26a对卵巢癌SKOV3细胞增殖及侵袭能力的影响。方法将卵巢癌SKOV3细胞分为miR-26a抑制剂组、miR-26a模拟物组和对照组,分别将miR-26a抑制剂或miR-26a模拟物转染至miR-26a抑制剂组或miR-26a模拟物组细胞,对照组细胞转染无关序列,Real-time PCR法检测各组细胞miR-26a表达水平,分别用MTT方法与Transwell细胞侵袭实验检测转染后各组SKOV3细胞的增殖与侵袭能力变化。Real-time PCR与Western blot实验分别检测各组细胞GSK-3βm RNA和蛋白表达水平。结果与对照组相比,miR-26a抑制剂组SKOV3细胞miR-26a表达水平显著降低,miR-26a模拟物组则显著升高(P<0.01)。与对照组相比,miR-26a抑制剂组SKOV3细胞增殖与侵袭能力显著降低,GSK-3βm RNA及蛋白表达水平显著升高,miR-26a模拟物组SKOV3细胞增殖与侵袭能力显著升高,GSK-3βm RNA及蛋白表达水平显著降低(P<0.01)。结论 miR-26a促进卵巢癌SKOV3细胞的增殖和侵袭可能与下调GSK-3β的表达相关。 Objective To investigate the effect of miR-26a on the proliferation and invasion of ovarian cancer SKOV3 cells. Methods SKOV3 cells were divided into miR-26a inhibitor group, miR-26a mimics group and control group. The miR-26a inhibitor or miR-26a mimics were transfected into miR-26a inhibitor group or miR- 26a mock cells and control cells were transfected with unrelated sequences. Real-time PCR was used to detect the expression of miR-26a in each group. The proliferation and invasion of SKOV3 cells in each group were detected by MTT assay and Transwell invasion assay Ability changes. Real-time PCR and Western blot were used to detect the expression of GSK-3βmRNA and protein in each group. Results Compared with the control group, miR-26a expression was significantly decreased in miR-26a inhibitor SKOV3 cells and significantly increased in miR-26a mimics (P <0.01). Compared with the control group, the proliferation and invasion ability of SKOV3 cells in miR-26a inhibitor group were significantly decreased, the expression of GSK-3βmRNA and protein were significantly increased, and the proliferation and invasion ability of SKOV3 cells was significantly increased in miR-26a mimics group. GSK -3βmRNA and protein expression levels were significantly lower (P <0.01). Conclusion miR-26a may promote the proliferation and invasion of ovarian cancer SKOV3 cells, which may be related to the down-regulation of GSK-3β expression.