1、前言有关微生物,特别是菌类遗传资源保存中的菌株保存,经过很多研究,出已成为可能。保存方法有继代培养法、流动石蜡复层法、冻结保存法、冻结干燥法、L—干燥法、蒸馏水保存法、土壤或砂保存法、硅胶保存法、园盘干燥保存法等,这些方法有许多变化发展,其中冻结保存法,在不同温度保存时,有的从—10℃到液氮温度(—196℃),有的加入甘油等保护剂 1, Preface The preservation of microorganisms, especially strains of germ genetic resources, has become possible after many studies. Methods of preservation include subculture, flow paraffin coating, freeze storage, freeze drying, L-drying, distilled water preservation, soil or sand preservation, silica gel preservation, disk dry preservation, etc. These methods There are many changes in the development, including frozen preservation method, at different temperatures, some from -10 ℃ to liquid nitrogen temperature (-196 ℃), and some glycerol and other protective agent