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目的:探讨模拟失重环境下大鼠血浆蛋白质组变化特征。方法:健康成年雄性Wistar大鼠88只,按模拟失重时相随机分为11组,分别为6 h、12 h、1 d、2 d、3 d、5 d、1周、2周、3周、4周及0 h组(对照组)。采用尾悬吊法建立模拟失重动物模型,实验结束时取动物静脉血,利用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术及MB-WCX磁珠检测大鼠静脉血浆蛋白质谱,应用Ciphergen Protein Chip Software 3.2.0和Biomarker Wizard 3.1.0软件分析数据。结果:发现18个重力敏感蛋白,其中在模拟失重早期,相对分子质量较小的6个蛋白的表达呈上调趋势,而相对分子质量较大的12个蛋白的表达则逐渐下调;在模拟失重后期(悬尾2~3周后),上述蛋白的表达均呈回归趋势。结论:模拟失重环境对大鼠静脉血浆蛋白质谱产生明显影响,研究重力敏感蛋白对进一步揭示失重对机体的影响及机制具有重要意义,并对医监医保可能有一定的价值。
Objective: To investigate the changes of plasma proteome in rats under simulated weightlessness. Methods: A total of 88 healthy adult male Wistar rats were randomly divided into 11 groups according to simulated weightlessness phase: 6 h, 12 h, 1 d, 2 d, 3 d, 5 d, 1 week, 2 weeks, 3 weeks , 4 weeks and 0 h group (control group). Animal models of simulated weightlessness were established by tail-suspension method. Venous blood was taken from the animals at the end of the experiment. The venous plasma protein profiles were detected by SELDI-TOF-MS and MB-WCX magnetic beads. Data was analyzed using Ciphergen Protein Chip Software 3.2.0 and Biomarker Wizard 3.1.0 software. RESULTS: Eighteen gravimetric-sensitive proteins were found. Among them, the expression of six proteins with relatively small molecular weight was up-regulated in early stage of simulated weightlessness, while the expression of twelve proteins with relatively high molecular weight was gradually decreased. In the period of simulated weightlessness (2-3 days after tail suspension), the expression of these proteins showed a regression trend. CONCLUSION: The simulated weightlessness environment has a significant effect on the venous plasma protein profile of rats. It is of great significance to study the effect of gravity-sensitive proteins on further revealing the effects of weightlessness on the body and its mechanism, and may be of some value to medical supervision.