葡萄亚属间(V.vinifera×V.rotundifolia)杂种胚挽救成苗的研究

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利用欧洲葡萄(V.vinifera)的无核品种与圆叶葡萄(V.rotundifolia)杂交的目的是将无核性状导入抗病的圆叶葡萄中以获得抗病无核葡萄新材料。本文利用无核材料P79-101(V.vinifera)与圆叶葡萄的3个株系杂交后,共产生102个杂种胚珠,这些胚珠经培养,有44个杂种胚得以继续发育,并获得胚挽救苗20株。经同工酶鉴定及植物形态特征分析,证明这20株苗中有19株为亚属间杂种苗。其中16株苗栽植于加州大学Fresno分校的试验田里。从败育的种子痕迹大小及浆果特点来看,其中C41-5株系属假单性结实的无核类型,它的果实比汤姆逊无核(Thompson Seedless)略小,是“黑可因”(Black Corinth)及C41-7果实的两倍。C41-5种子痕迹的重量比汤姆逊无核的大,但两者无显著差异;而C41-7发育完全的种子平均重量是C41-5种子平均重量的10倍。本文对V.vinifera×V.rotundifolia亚属间杂种胚成功的挽救进行了首次报道。 The purpose of the hybrid with V. rotundifolia, a non-nuclear variety of V. vinifera, is to introduce seedless kernels into disease-resistant leafy grapes to obtain new materials resistant to diseaseless grapes. In this study, 102 hybrid ovules were produced after hybridization of P79-101 (V.vinifera) with three lines of V. mellifera. These embryos were cultured and 44 hybrid embryos were allowed to continue to develop and embryo rescue 20 seedlings. Isozyme identification and plant morphological characteristics analysis showed that 19 out of 20 seedlings were inter-subspecific hybrid seedlings. Sixteen of these were planted in experimental fields at the University of California at Fresno. In terms of the size of the aborted seeds and the characteristics of the berries, the C41-5 strain is a pseudo-unisexual seedless type with a slightly smaller fruit than Thompson Seedless, Twice the Black Corinth and C41-7 fruits. The weight of C41-5 seeds was larger than that of Thomson seeds, but there was no significant difference between them. However, the average weight of C41-7 seeds was 10 times that of C41-5 seeds. This paper reports the first successful rescue of V.vinifera × V.rotundifolia intersubject hybrid embryos.
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