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采用聚电解质自组装技术制备局域表面等离子体共振(LSPR)传感膜的方法,在玻璃基片上依次沉积聚电解质PDDA,PSS和PVTC,并通过静电吸附构建胶体金纳米粒子自组装膜形成LSPR传感膜.利用扫描电镜对LSPR传感膜表面形貌以及膜中金纳米粒子的粒径进行了表征,同时通过紫外-可见消光光谱对其灵敏度和渗透深度等重要参数进行检测.研究结果表明,所制备的LSPR传感膜粒子分布均匀、单分散性好、稳定性高、重现性好;消光峰位对样品溶液折射率的检测灵敏度为71nm/RIU,相应的峰强检测灵敏度为0.21AU/RIU,对表面吸附层的渗透深度约为16nm.
The method of preparing localized surface plasmon resonance (LSPR) sensing film by using polyelectrolyte self-assembly technology is that the polyelectrolyte PDDA, PSS and PVTC are sequentially deposited on a glass substrate, and the self-assembling film of colloidal gold nano-particles is formed by electrostatic adsorption to form the LSPR Sensing film.The surface morphology of LSPR sensing film and the size of gold nanoparticles in the film were characterized by scanning electron microscopy, and the important parameters such as sensitivity and penetration depth were detected by UV-visible spectroscopy.The results show that , The prepared LSPR sensing film has the advantages of uniform particle distribution, good monodispersity, high stability and good reproducibility. The detection sensitivity of the extinction peak to the refractive index of the sample solution is 71 nm / RIU and the corresponding peak intensity detection sensitivity is 0.21 AU / RIU, the penetration depth of the surface adsorption layer is about 16 nm.