论文部分内容阅读
[目的]探讨不同时间点给予促红细胞生成素(EPO)对戊四氮(PTZ)致癫幼鼠癫痫(EP)后的脑保护作用。[方法]60只SD幼鼠随机分为6组,实验组(A～E组)注射PTZ50mg/(kg.次),对照组(F组)注射等量生理盐水。实验组分为单纯致癫组(A组)及EPO干预组(B～E组),于癫痫发作前24h(B组)、发作后0h(C组)、6h(D组)、9h(E组),分别注射rhEPO5000u/(kg.次);均于EP后24h取海马,用酶联免疫吸附法检测神经元特异性烯醇酶(NSE)和S100β水平。[结果]B组癫痫发作程度及严重性较A组减轻。海马NSE及S100β浓度A、E组均高于B、C、D和F组,而B、C、D组与F组间类似。[结论]EPO可能具有抗癫痫作用,对PTZ致痫幼鼠具神经保护作用,尤以EP发生后6h内给药作用最佳。
[Objective] To explore the protective effect of erythropoietin (EPO) on epilepsy (EP) induced by pentylenetetrazole (PTZ) in rats at different time points. [Method] Sixty young SD rats were randomly divided into 6 groups. The experimental group (group A ~ E) received 50 mg / (kg) PTZ injection and the control group (group F) received the same amount of saline. The experimental group consisted of epilepsy group (group A) and EPO intervention group (group B ~ E) at 24 h before seizure (group B), 0 h after seizure (group C), 6 h Group) were injected rhEPO5000u / (kg. Times); hippocampus were harvested 24 hours after EP, and neuron-specific enolase (NSE) and S100β levels were detected by enzyme-linked immunosorbent assay. [Results] The seizure severity and severity of group B were less than those of group A. The concentrations of NSE and S100β in hippocampus were higher in groups A, E than in groups B, C, D and F, while those in groups B, C and D were similar to those in group F. [Conclusion] EPO may have antiepileptic effect and have neuroprotective effects on young rats with PTZ-induced epilepsy, especially in the 6h after EP.