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马铃薯在生长发育过程中受各种生物和非生物胁迫的影响,干旱是其中最常见和危害最严重的非生物胁迫因素之一,常常导致单产不高,总产不稳,严重影响着马铃薯产业的发展。本研究以改善马铃薯抗旱性为目的,采用PCR方法从拟南芥中克隆了转录因子DREB1A基因和诱导型启动子rd29A。利用DNA重组技术成功构建了诱导型启动子rd29A驱动转录因子DREB1A基因和CaMV35S启动子驱动Bar基因的双价植物表达载体pBI121-rd29-BDR,并通过农杆菌介导法对马铃薯进行遗传转化,PPT筛选得到22株抗性苗,PCR和RT-PCR检测证明DREB1A基因已整合到陇薯10号马铃薯基因组中并在转基因植株中转录表达,有望提高转基因马铃薯的抗旱性。目前,作者正在进行转基因马铃薯的抗旱性分析研究。
Potato is under the influence of various biological and abiotic stresses during its growth and development. Drought is one of the most common and harmful abiotic stress factors, which often leads to low yield and unstable yield, which seriously affects the potato industry development of. In order to improve the drought resistance of potato, we cloned the transcription factor DREB1A and the inducible promoter rd29A from Arabidopsis using PCR. The recombinant expression vector pBI121-rd29-BDR was constructed by DNA recombination technology, which was driven by the inducible promoter rd29A driven transcription factor DREB1A gene and the CaMV35S promoter driven Bar gene. The transgenic plants were transformed by Agrobacterium tumefaciens method. PPT Twenty-two resistant seedlings were screened. The results of PCR and RT-PCR showed that the DREB1A gene was integrated into the potato genome of Longshu 10 and transcribed into the transgenic plants. It is expected to improve the drought resistance of transgenic potato. At present, the author is carrying out drought resistance analysis of transgenic potato.