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本文研究苯并(a)芘(B[a]P)对发育鸡胚的毒效应。将白色莱亨鸡蛋置于38℃和60～65%相对湿度的孵卵器中。处理前将全部蛋对光检查,仅选重55～60 g、具有活胚胎的受精卵用于本研究。每组用蛋40只。于孵化的第4～9天,向发育的胚胎的卵黄囊中,无菌注射溶于20μl花生油的5、50和100μg/蛋剂量的B[a]P,对照组注射20μl花生油。孵育20天后,破壳检查雏鸡的存活和异常。
This article studies the toxic effect of benzo (a) pyrene (B [a] P) on developing chicken embryos. White Leheng eggs were placed in an incubator at 38 ° C and 60-65% relative humidity. All eggs were examined for light before treatment, weighing only 55-60 g. Fertilized eggs with live embryos were used in this study. 40 eggs per group. From day 4 to day 9 of hatching, to the developing yolk sac of the embryos, 5 [mu] g and 50 [mu] g / egg dose of B [a] P in 20 [mu] l peanut oil were aseptically injected while the control group was injected with 20 [mu] l peanut oil. After 20 days of incubation, the chickens were checked for survival and abnormalities.