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为了解肿瘤抑制基因ＡＰＣ基因在大肠癌中突变的规律。方法应用聚合酶链反应┐单链构象多态性技术（ＰＣＲ┐ＳＳＣＰ）对３０例人大肠癌组织ＡＰＣ基因１５号外显子的三个区１５┐６、１５┐７、１５┐８进行了检测。检测步骤主要包括人基因组ＤＮＡ提取、ＰＣＲ反应扩增及ＰＣＲ产物的ＳＳＣＰ分析。结果检查结果显示在第１５┐６及１５┐７片段上共１０例发生了突变，突变率为３３．３％。突变的１０例中有８例发生于１５┐７外显子片段，占突变总数８０％（８／１０），２例发生于１５┐６片段，占突变总数的２０％（２／１０）。结论证明我国大肠癌患者中存在着ＡＰＣ基因的突变，且１５┐７片段是突变集中区。
To understand the law of mutation of the tumor suppressor gene APC gene in colorectal cancer. Methods Polymerase chain reaction single strand conformation polymorphism (PCR┐SSCP) was used to detect the three regions 15156,15┐7,15┐8 of exon 15 of APC gene in 30 human colorectal cancer tissues. . The detection steps mainly include human genomic DNA extraction, PCR amplification, and SSCP analysis of PCR products. The results of the examination showed that a total of 10 cases had mutations on the 15th and 6th fragments, and the mutation rate was 33.3%. Eight of the 10 mutations occurred in the 15┐7 exon fragment, accounting for 80% (8/10) of the mutations, and 2 cases occurred in the 15┐6 fragment, accounting for 20% (2/10) of the total mutations. Conclusion It is proved that there is a mutation in APC gene in patients with colorectal cancer in China, and the 15┐7 fragment is the mutation concentration area.