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以组织培养和细胞液印迹直接荧光观察法相结合，建立了伊贝母组织培养生物合成高含量贝母碱的技术，并通过薄层层析和薄层扫描对贝母生物碱的组成进行了比较分析。结果表明，不论是组织培养物还是栽培贝母中都含有贝母碱的主要成分——西贝素。但根中贝母碱的成分要比其它组织或器官中更为复杂，含量和活性也较高。继代培养８代的不定芽和不定根中贝母碱的含量分别高于栽培５年贝母根的０．０１％和０．０２％。
Based on the combination of tissue culture and direct fluorescent observation of cytosol, a technique was established for the biosynthesis of Fritillaria in Iberian tissue, and the composition of alkaloids in Fritillaria was compared by TLC and TLC analysis. The results showed that both the tissue culture and the cultivated fritillaria contain the main component of felbinar, However, the composition of Fritillaria root than other tissues or organs more complex, higher content and activity. Adventitious buds subcultured for 8 generations and Fritillaria adventitious root content were higher than 0.01% and 0.02% of cultivated 5-year Fritillaria.