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将45只Wistar大鼠随机分为对照组、低氟组和高氟组,每组15只。对照组大鼠给予高脂饮食4周,一次性腹腔注射50 mg/kg的链脲佐菌素(STZ),复制2型糖尿病(T2DM)大鼠模型,继续喂养12周,每天灌胃0.1 ml/kg生理盐水。低氟、高氟组大鼠每天分别灌胃给予10 mg/kg、20mg/kg含氟水,连续16周,其余措施同对照组。比较三组大鼠肝脏组织匀浆中氧化应激指标丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)及总抗氧化能力(T-AOC)的变化。结果显示,低氟组和高氟组肝组织匀浆MDA、NO均明显高于对照组(P<0.05),SOD、CAT、GSH及T-AOC均明显低于对照组(P<0.05);而以高氟组更加明显,与低氟组比较,差异也有统计学意义(P<0.05)。提示氟能增加STZ诱导的T2DM大鼠肝脏氧化应激损伤,降低肝脏抗氧化因子水平。
Forty-five Wistar rats were randomly divided into control group, low fluoride group and high fluoride group, 15 rats in each group. Rats in the control group were given a high-fat diet for 4 weeks and were given a single intraperitoneal injection of streptozotocin (STZ) at a dose of 50 mg / kg. The model rats with type 2 diabetes mellitus (T2DM) were injected intraperitoneally for 12 weeks. / kg saline. The rats in low fluoride group and high fluoride group were given gavage with 10 mg / kg, 20 mg / kg fluoride water respectively for 16 consecutive weeks. The other measures were the same as the control group. The levels of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), reduced glutathione Peptide (GSH) and total antioxidant capacity (T-AOC) changes. The results showed that the content of MDA and NO in the liver homogenate of the low fluoride group and the high fluoride group were significantly higher than those of the control group (P <0.05), and the levels of SOD, CAT, GSH and T-AOC were significantly lower than those of the control group (P <0.05). In the high fluoride group, the difference was statistically significant (P <0.05). Tip fluoride can increase STZ-induced liver injury in T2DM rats oxidative stress, reduce liver anti-oxidant levels.