【摘 要】
：
建立高效液相色谱荧光检测法同时检测鸡组织中阿莫西林和氨苄西林残留的方法.鸡组织样品经磷酸二氢钠溶液提取,乙腈去蛋白,正己烷脱脂,饱和二氯甲烷萃取,上清液经水杨醛在酸
【机 构】
：
扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,南京农业大学动物医学院
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建立高效液相色谱荧光检测法同时检测鸡组织中阿莫西林和氨苄西林残留的方法.鸡组织样品经磷酸二氢钠溶液提取,乙腈去蛋白,正己烷脱脂,饱和二氯甲烷萃取,上清液经水杨醛在酸性条件下沸水浴衍生化.以C18柱为固定相、0.01 mol/L的磷酸二氢钾溶液和乙腈溶液为流动相,梯度洗脱,流速为1 mL/min,荧光检测激发波长为358舢,发射波长为440 nm.阿莫西林和氨苄西林在25～1 000 μg/kg体重范围内线性关系良好,相关系数均大于0.9994.当阿莫西林和氨苄西林添加水平在10～100 μg/kg体重时,鸡组织样品中平均回收率均高于68.65％,相对标准偏差均低于10.74％.阿莫西林、氨苄西林在鸡组织中的检测限均分别为5、3.5 μg/kg体重,阿莫西林、氨苄西林的定量限均分别为13、10μg/g体重.该方法灵敏、准确、快速,适用于鸡组织中阿莫西林、氨苄西林残留的同时检测.
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