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在包括食管癌在内的多种肿瘤中,Plk1呈现过表达且具重要预后价值,表明该分子是一个潜在的肿瘤治疗靶点.为了探讨Plk1作为分子靶点用于食管癌治疗的潜在价值,采用siRNA技术抑制Plk1的表达,并观察了其对3株代表性食管癌细胞生长的影响.结果表明:合成的特异性短链Plk1 siRNA在mRNA和蛋白质水平上均能高效、特异性地抑制3株代表性食管癌细胞系中Plk1的表达.同时,siRNA介导的Plk1表达沉默显著抑制了食管癌细胞的生长,细胞活力同时显著下降.另外,Plk1表达被抑制后,大量细胞有丝分裂受到阻滞,并进而发生大规模细胞死亡.这些结果表明,siRNA介导的Plk1表达沉默能显著抑制食管癌细胞的生长,Plk1是一个潜在的用于食管癌治疗的新靶点.
Plk1 is overexpressed and has a prognostic value in many tumors, including esophageal cancer, indicating that this molecule is a potential target for oncology.To explore the potential value of Plk1 as a molecular target for the treatment of esophageal cancer, The siRNA technique was used to inhibit the expression of Plk1 and its effect on the growth of three representative esophageal cancer cells was observed.The results showed that the synthesized specific short chain Plk1 siRNA could efficiently and specifically inhibit the expression of Plk1 at mRNA and protein levels Strain Plk1 expression in esophageal cancer cell lines.At the same time, silencing of Plk1 mediated by siRNA significantly inhibited the growth and cell viability of esophageal cancer cells.At the same time, Plk1 expression was inhibited, and a large number of cell mitosis were blocked , And then a large-scale cell death.These results suggest that siRNA-mediated Plk1 silencing can significantly inhibit the growth of esophageal cancer cells, Plk1 is a potential new target for the treatment of esophageal cancer.