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目的:研究人巨细胞病毒(HCMV)UL145序列在先天感染患儿临床株中的基因多态性,探讨HCMV基因多态性与先天感染引起的不同临床症状之间的关系。方法:对16株临床低传代分离株和15株未传代临床株的HCMV临床标本分别进行UL145全序列PCR扩增,对PCR扩增阳性的31例标本进行序列测定及分析,并且与9株已在GenBank递交的HCMV UL145序列进行比较分析。结果:序列分析结果表明31株HCMV临床株的UL145基因是高度保守的。所有临床株的HCMV UL145开放阅读框架均为393 bp,编码蛋白含有130个氨基酸。所有临床株的核苷酸同源率为95.9%～100%,编码蛋白的同源率为97.7%～100%。临床症状不同的患儿其HCMV UL145基因及其编码蛋白具有相似的结构。所有先天感染患儿临床株的UL145编码蛋白具有蛋白激酶(C PKC)磷酸化功能位点和酪蛋白激酶(CK2)磷酸化功能位点。结论:HCMV UL145基因在临床株中是高度保守,未发现其与HCMV先天感染不同临床症状间存在明显的关系。HCMV UL145基因的高度保守性在先天感染中具有重要作用。
Objective: To study the genetic polymorphism of human cytomegalovirus (HCMV) UL145 sequence in children with congenital infection and explore the relationship between HCMV gene polymorphism and different clinical symptoms caused by congenital infection. Methods: The clinical specimens of 16 low-passage clinical isolates and 15 non-passaged clinical isolates of HCMV were respectively subjected to full-length UL145 PCR amplification. Sequencing and analysis of 31 samples with positive PCR amplification were carried out. HCMV UL145 sequences submitted in GenBank were analyzed comparatively. Results: Sequence analysis showed that the UL145 gene of 31 HCMV clinical strains was highly conserved. The HCMV UL145 open reading frame of all clinical isolates was 393 bp, and the encoded protein contained 130 amino acids. The nucleotide homology of all clinical strains was 95.9% ~ 100%, and the homology of the encoded proteins was 97.7% ~ 100%. Children with different clinical symptoms of HCMV UL145 gene and its encoded protein has a similar structure. The UL145-encoded protein from clinical strains of children with congenital infection possesses both a protein kinase (C PKC) phosphorylation site and a casein kinase (CK2) phosphorylation site. CONCLUSION: The HCMV UL145 gene is highly conserved in clinical isolates and no significant association with different clinical symptoms of congenital HCMV infection was found. The high degree of conservation of HCMV UL145 gene plays an important role in congenital infection.