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目的探讨舒肌汤治疗帕金森病肌僵直的可能作用机理。方法采用颅内注射乳胞素诱导帕金森病肌僵直大鼠模型共24只,随机分为模型组和中药高、中、低剂量组,每组6只,另设假手术组6只(颅内注射生理盐水)。假手术组和模型组均灌胃生理盐水20 g/(kg·d),舒肌汤浓缩至2.0 g/ml,中药高、中、低剂量组分别灌服舒肌汤40、20、10 g/(kg·d),每日1次,持续灌胃30天。检测大鼠纹状体多巴胺(DA)、高香草酸(HVA)和二羟苯乙酸(DOPAC)的含量,电镜观察膈肌神经肌肉接头超微结构改变。结果与假手术组比较,模型组大鼠纹状体DA、HVA、DOPAC含量明显减少,DA/DOPAC和DA/HVA值下降(P<0.05或P<0.01);与模型组比较,中药中、低剂量组大鼠纹状体DA、HVA、DOPAC含量及DA/DOPAC和DA/HVA值均升高(P<0.05或P<0.01);与中药中剂量组比较,中药高剂量组DOPAC含量和DA/DOPAC、DA/HVA值下降,中药低剂量组DOPAC含量亦降低(P<0.05)。电镜下,模型组存在神经肌肉接头处肌纤维密集,排列紊乱,突触前膜囊泡和线粒体增多,突触后膜皱褶明显,乙酰胆碱受体增多等超微结构改变,中药各剂量组均能不同程度改善上述改变。结论舒肌汤可能通过促进DA分泌,同时减少神经肌肉接头处乙酰胆碱的释放,调节中枢和外周神经兴奋,从而缓解帕金森病肌僵直。
Objective To investigate the possible mechanism of Shuji decoction in treating Parkinson’s disease. Methods A total of 24 model rats with Parkinson’s disease were induced by intracerebral injection of lactacystin. The rats were randomly divided into model group and high, medium and low dose groups of Chinese medicine, with 6 rats in each group. Six rabbits Saline injection). Sham operation group and model group were given normal saline 20 g / (kg · d), Shu muscle soup concentrated to 2.0 g / ml, high, medium and low dose group were given Shu muscle soup 40,20,10 g / (kg · d), once daily, continued gavage for 30 days. The content of dopamine (DA), homovanillic acid (HVA) and dihydroxybenzene acetic acid (DOPAC) in the striatum of rats were detected. The ultrastructural changes of the neuromuscular junction of the diaphragm were observed by electron microscope. Results Compared with the sham operation group, the content of DA, HVA and DOPAC in the model group decreased significantly and the DA / DOPAC and DA / HVA values decreased (P <0.05 or P <0.01). Compared with the model group, The content of DA, HVA, DOPAC and DA / DOPAC and DA / HVA in the low dose group were significantly increased (P <0.05 or P <0.01). Compared with the medium dose group, DA / DOPAC, DA / HVA values decreased, and DOPAC content in low-dose Chinese medicine group also decreased (P <0.05). Under electron microscope, the model group had dense muscle fibers at the neuromuscular junction, disordered arrangement, increased presynaptic vesicles and mitochondria, obvious folds in the postsynaptic membrane and increased acetylcholine receptors, To varying degrees improve the above changes. Conclusions Shuyitang may relieve the stiffness of Parkinson’s disease by promoting the secretion of DA and at the same time reducing the release of acetylcholine at the neuromuscular junction and regulating the excitement of the central and peripheral nerves.