论文部分内容阅读
目的构建人WWOX基因真核细胞表达载体。方法采用RT-PCR方法,从人新鲜卵巢组织的总RNA中扩增出1245bp的人WWOXcDNA片段,然后用HindⅢ和EcoRI双酶切后定向克隆到真核细胞表达载体pcDNA3.1中,用限制性内切酶酶切分析和DNA序列分析鉴定重组质粒。结果WWOX基因的序列测定结果与文献报道完全一致,其真核细胞表达载体被成功构建。结论成功克隆出WWOX基因,并成功构建其真核细胞表达载体。
Objective To construct eukaryotic expression vector of human WWOX gene. METHODS: Human WWOX cDNA fragment of 1245bp was amplified by RT-PCR from total RNA of human ovarian tissue and then cloned into eukaryotic expression vector pcDNA3.1 by digestion with HindIII and EcoRI. Endonuclease digestion analysis and DNA sequence analysis identified the recombinant plasmid. Results The sequence of WWOX gene was completely consistent with that reported in the literature. The eukaryotic expression vector was successfully constructed. Conclusion The WWOX gene was successfully cloned and its eukaryotic expression vector was successfully constructed.