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目的:探讨舌鳞状细胞癌组织中hTERT与PTEN蛋白表达的相关关系,为治疗原发性舌鳞癌提供理论依据。方法:采用免疫组织化学技术及原位杂交技术,对原发性舌鳞癌组织、癌旁组织(正常组织)中hTERT和PTEN蛋白表达进行检测。利用计算机图像分析技术,将二者中hTERT和PTEN蛋白水平进行半定量分析。在此基础上,应用有关统计学方法,评价hTERT与PTEN之间是否存在相关性及其相关程度。结果:舌鳞癌中hTERT和PTEN蛋白的阳性检出率分别为74.5%(37/50)和39.1%(20/50)。且随着肿瘤恶性程度的增加,hTERT的表达升高,PTEN的表达下降,二者之间存在相关性(P<0.01)。结论:抑癌基因PTEN在舌鳞状细胞癌中对hTERT活化的可能存在抑制作用,PTEN的缺失可能引起hTERT表达水平增高从而导致舌癌的发生。
Objective: To investigate the relationship between hTERT and PTEN protein expression in tongue squamous cell carcinoma and to provide a theoretical basis for the treatment of primary tongue squamous cell carcinoma. Methods: Immunohistochemistry and in situ hybridization were used to detect the expression of hTERT and PTEN protein in primary tongue squamous cell carcinoma and paracancerous tissues (normal tissues). Semi-quantitative analysis of hTERT and PTEN protein levels was performed using computer image analysis techniques. On this basis, the use of statistical methods to evaluate the correlation between hTERT and PTEN and their relevance. Results: The positive rates of hTERT and PTEN in tongue squamous cell carcinoma were 74.5% (37/50) and 39.1% (20/50), respectively. The expression of hTERT increased and the expression of PTEN decreased as the degree of malignancy increased (P <0.01). CONCLUSION: PTEN suppresses the activation of hTERT in tongue squamous cell carcinoma. The loss of PTEN may result in the increase of expression of hTERT, leading to the occurrence of tongue cancer.