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目的 :研究温补培元方治疗脾虚证作用机制。方法 :利用大黄制成的大鼠脾虚模型 ,并用从新安著名温补培元医家案中总结出的温补培元方进行治疗 ,在电镜下观察其小肠肌间神经丛胆碱能神经终末的清亮小泡的数量 ,运用立体定量分析法进行计算。结果 :发现用药组 ACh终末的清亮小泡数明显低于对照组 (P<0 .0 5 )。结论 :提示该方有明显减少清亮小泡的数量 ,抑制胆碱能神经功能亢进作用 ,从而改善大鼠的脾虚证候
Objective: To study the mechanism of Wenpi Peiyuan Prescription on the treatment of spleen deficiency syndrome. METHODS: The model of spleen deficiency in rats made from rhubarb was treated with the Wenbu Peiyuan prescription, which was summed up from the well-known Wenpai Peiyuan doctors’ case in Xinan. The cholinergic nerve terminals in the myenteric plexus of the small intestine were observed under electron microscope. The number of clear vesicles was calculated using a three-dimensional quantitative analysis method. RESULTS: The number of clear vesicles at the end of ACh in the drug group was significantly lower than that in the control group (P<0.05). Conclusion : It is suggested that the number of clear vesicles is significantly reduced and the cholinergic nerve function is inhibited by this side, thus improving the spleen deficiency syndrome in rats.