论文部分内容阅读
目的采用反相HPLC法同时测定广金钱草中vicenin-1、夏佛塔苷和vicenin-3的含量。方法采用Kromasil RPC18色谱柱(250 mm×4.6 mm,5μm),柱温30℃,流动相A相为水-0.1%甲酸水溶液、B相为甲醇,梯度洗脱:0~8 min、30%~32%B,8~32 min、32%~34%B,32~60 min、34%~40%B,流速1.0 mL·min-1,检测波长272 nm。结果 vicenin-1在0.230~1.380μg(r=0.9998)、夏佛塔苷在0.702~1.872μg(r=0.9998)、vicenin-3在0.220~1.320μg(r=0.9999)的线性关系良好;平均回收率(n=6)分别为99.44%、99.58%、100.01%。结论所用方法具有简便、快速准确、重复性好的特点,可为广金钱草药材的质量控制提供依据。
OBJECTIVE To determine the content of vicenin-1, chardoside and vicenin-3 in Desmodium Styracifolium by reverse phase HPLC. Methods A Kromasil RPC18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase A was water-0.1% formic acid aqueous solution and the mobile phase A was methanol. The mobile phase A was eluted with gradient elution from 0 to 8 min and 30% 32% B, 8 ~ 32 min, 32% ~ 34% B, 32 ~ 60 min, 34% ~ 40% B at a flow rate of 1.0 mL · min- Results The linear relationship between vicenin-1 and 0.220 ~ 1.320μg (r = 0.9999) was good at 0.230 ~ 1.380μg (r = 0.9998) Rates (n = 6) were 99.44%, 99.58%, 100.01%, respectively. Conclusion The method is simple, rapid and accurate, good reproducibility, which can provide the basis for the quality control of Medicinal Herbal Medicine.